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Asian J Beauty Cosmetol > Accepted Articles

doi: https://doi.org/10.20402/ajbc.2017.0188    [Accepted]
Anti-oxidant Effects of Hydroxytyrosol in Human Dermal Fibroblasts
Mi-Na Choi1, Eun-Ju Na2, In-Sook An3, Sung-Nae Lee4, Young-Sam Kim5
1Department of Liberal Arts, Jang-An University, Hwaseong-si, Gyeonggi-do, Korea
2Department of Biological Engineering, Konkuk University, Seoul, Korea
3GeneCellPharm Corporation, Seoul, Korea
4Department of Cosmetology, Kyung-In Women’s University, Incheon, Korea
5Department of Image Industry, Graduate School of Engineering, Konkuk University, Seoul, Korea
Corresponding author:  Young-Sam Kim ,Tel: +82 2 450 3595, Email: gracehelen@konkuk.ac.kr
Received: September 6, 2017;  Accepted: November 20, 2017.  Published online: November 22, 2017.
ABSTRACT
Purpose:
Hydroxytyrosol is a kind of phenolic plant chemicals with anti-oxidant properties. In this study, we investigated intracellular mechanism of hydroxytyrosol in human dermal fibroblasts (HDFs) induced by ultraviolet A (UVA) and hydroxytyrosol in human dermal fibroblasts.
Method:
The quantitative real-time PCR (qRT-PCR) was used to quantitatively analyze the gene expression changes in HDFs by hydroxytyrosol. The cell survival rate was measured using the principle of WST-1 assay, which is a measurement method for cell survival rate. To demonstrate the anti-oxidative effects of hydroxytyrosol, quantitative analysis of ROS and changes of GSH were measured using DCF-DA, and the expression of GPx1, SOD1 and CAT genes was examined.
Results:
Cell viability decreased by UVA was increased by hydroxytyrosol concentration. In the treatment of hydroxytyrosol with UVA, the anti-oxidant effects were confirmed by the reduction of ROS scavenging and GSH level, and the decrease of GPx1, SOD1 and CAT mRNA expression.
Conclusion:
Through this research, we demonstrate that hydroxytyrosol has anti-oxidant effects in HDFs damaged by UVA. We suggest that hydroxytyrosol is fully worthy of using as a cosmetic material effective to anti-oxidants on HDFs.
Key words: Hydroxytyrosol, Anti-oxidant, HDFs, Ultraviolet A, Cosmetic
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