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Asian J Beauty Cosmetol > Accepted Articles

doi: https://doi.org/10.20402/ajbc.2017.0188    [Accepted]
Anti-oxidant Effects of Hydroxytyrosol in Human Dermal Fibroblasts
Mi-Na Choi1, Eun-Ju Na2, In-Sook An3, Sung-Nae Lee4, Young-Sam Kim5
1Department of Liberal Arts, Jang-An University, Hwaseong-si, Gyeonggi-do, Korea
2Department of Biological Engineering, Konkuk University, Seoul, Korea
3GeneCellPharm Corporation, Seoul, Korea
4Department of Cosmetology, Kyung-In Women’s University, Incheon, Korea
5Department of Image Industry, Graduate School of Engineering, Konkuk University, Seoul, Korea
Corresponding author:  Young-Sam Kim ,Tel: +82 2 450 3595, Email: gracehelen@konkuk.ac.kr
Received: September 6, 2017;  Accepted: November 20, 2017.  Published online: November 22, 2017.
Hydroxytyrosol is a kind of phenolic plant chemicals with anti-oxidant properties. In this study, we investigated intracellular mechanism of hydroxytyrosol in human dermal fibroblasts (HDFs) induced by ultraviolet A (UVA) and hydroxytyrosol in human dermal fibroblasts.
The quantitative real-time PCR (qRT-PCR) was used to quantitatively analyze the gene expression changes in HDFs by hydroxytyrosol. The cell survival rate was measured using the principle of WST-1 assay, which is a measurement method for cell survival rate. To demonstrate the anti-oxidative effects of hydroxytyrosol, quantitative analysis of ROS and changes of GSH were measured using DCF-DA, and the expression of GPx1, SOD1 and CAT genes was examined.
Cell viability decreased by UVA was increased by hydroxytyrosol concentration. In the treatment of hydroxytyrosol with UVA, the anti-oxidant effects were confirmed by the reduction of ROS scavenging and GSH level, and the decrease of GPx1, SOD1 and CAT mRNA expression.
Through this research, we demonstrate that hydroxytyrosol has anti-oxidant effects in HDFs damaged by UVA. We suggest that hydroxytyrosol is fully worthy of using as a cosmetic material effective to anti-oxidants on HDFs.
Key words: Hydroxytyrosol, Anti-oxidant, HDFs, Ultraviolet A, Cosmetic
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