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Asian J Beauty Cosmetol > Accepted Articles

doi: https://doi.org/10.20402/ajbc.2017.0183    [Accepted]
Inhibition of Apoptosis and Anti- inflammatory Effects of Embelin
Hee-Jung Yong1,2, Gyu Ri Kim4, Jin-Jung Ahn5, In-Sook An3, Yong-Sam Kim
1Department of Lecture Beauty People Beauty School , Seoul, Korea
2Department of Bioengineering, Konkuk University, Seoul, Korea
3Department of Beauty Arts, Suwon Women’s University, Suwon-si, Gyeonggi-do, Korea
4GeneCellPharm Corporation, Seoul, Korea
5Department of Image Industry, Graduate School of Engineering, Konkuk University, Seoul, Korea
Corresponding author:  In-Sook An ,Tel: +82 2 450 3595, Email: gracehelen@konkuk.ac.kr
Received: September 1, 2017;  Accepted: November 20, 2017.  Published online: November 22, 2017.
Embelin is a major active ingredient of Embelia ribes Burm, belonging to Myrsinaceae, and an important traditional medicinal plant of Indian origin. Embelin has been studied for anti-cancer, anti-inflammatory, and anti-oxidative effects. However, there have been no studies on the use of cosmetic raw materials for embelin. Therefore, in this study, we investigated the inhibition of apoptosis and the anti-inflammatory effect to confirm the possibility of embelin as a cosmetic material.
Cell viability was measured by using water-soluble tetrazolium salt (WST-1) assay. Quantitative real time polymerase chain reaction (qRT-PCR) was performed to quantitatively analyze the gene expression patterns of embelin-induced inhibition of apoptosis and anti-inflammatory effects in human dermal fibroblasts (HDFs). Apoptosis ELISA kit was used to confirm the degree of apoptosis. 5,5 ', 6,6'-tetrachloro-1,1', 3,3'-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) mitochondrial membrane potential detection kit was used to measure mitochondrial membrane potential.
The WST-1 assay confirmed that the reduced cell viability by hydrogen peroxide (H2O2) was restored by embelin in a concentration-dependent manner. H2O2-induced apoptosis was restored by embelin, BAX gene expression was decreased and mitochondrial membrane potential difference was increased in a concentration dependent manner. It was confirmed that embelin inhibits apoptosis in HDFs treated with H2O2. In order to investigate the anti-inflammatory effect of embelin in H2O2-treated human dermal fibroblasts, gene expression of cyclooxygenase 2 (COX2), tumor necrosis factor  (TNF), and interleukin 6 (IL6), which induce inflammation, was examined and found to be decreased by embelin in a concentration dependent manner.
These results suggest that embelin has a potential as an anti-aging cosmetic ingredient with inhibition of apoptosis and anti-inflammatory properties.
Key words: Embelin, Apoptosis, Anti-inflammatory, Fibroblast, Hydrogen peroxide
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