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Asian J Beauty Cosmetol > Accepted Articles

doi: https://doi.org/10.20402/ajbc.2017.0162    [Accepted]
Human Dermal Stem/Progenitor Cell-derived Conditioned Medium Ameliorates Ultraviolet A-induced Damage of Normal Human Epidermal Keratinocytes
Joong Hyun Shim
Faculty of Cosmetics and Beauty Biotechnology, Semyung University, Jecheon-si, Chungcheongbuk-do, Korea
Corresponding author:  Joong Hyun Shim ,Tel: 82-43-649-1615, Fax: 82-43-649-1730, Email: jhshim@semyung.ac.kr
Received: July 7, 2017;  Accepted: October 30, 2017.  Published online: February 13, 2018.
ABSTRACT
Purpose:
The present study aimed to define the epidermal moisturizing effects of human dermal stem/progenitor cell-derived conditioned medium (hDSPC-CM) on normal human epidermal keratinocytes (NHEKs).
Method:
To investigate the epidermal moisturizing effects of hDSPC-CM on NHEKs, I measured 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and gene expression and performed cell viability assay and hyaluronic acid (HA)–enzyme-linked immunosorbent assay (ELISA).
Results:
In the present study, I elucidated the effects of hDSPC-CM on the mRNA expression of hyaluronan synthase 2 (HAS2), aquaporin 3 (AQP3), keratin 1 (KRT1), and keratin 10 (KRT10) and HA production. Real-time polymerase chain reaction (real-time PCR) revealed that hDSPC-CM increased HAS2 and AQP3 expression in NHEKs, and HA-ELISA revealed that hDSPC-CM increased HA production in NHEKs. On the other hand, hDSPC-CM decreased KRT1 and KRT10 expression in NHEKs.
Conclusion:
The results revealed that hDSPC-CM is a potent moisturizing cosmetic ingredient for epidermal moisturization. Based on this study, I anticipate further studies on mechanisms underlying the effects of hDSPC-CM on epidermal keratinocytes to develop not only cosmetics but also healthcare medicines.
Key words: hDSPC-CM, Moisturizing, HAS2, AQP3, Hyaluronic acid
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