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Asian J Beauty Cosmetol > Accepted Articles

doi: https://doi.org/10.20402/ajbc.2017.0197    [Accepted]
Anti-wrinkle and Skin turnover Improvement Effects of Niacinamide-dipeptide Convergence
Ga Yeon Kim1, Seung Je Lee1, Mi Ji Jeon1, Bo Min Kim1, Geun Tae Kim1, Sang Moon Kang2, Kee Young Lee2, Eun Jin Shin2, Sang Yong Kim3, Young Min Kim1
1Department of Biological Sciences and Biotechnology, Hannam University, Daejeon, Korea
2A&PEP Affiliated R&D Center, Cheongju-si, Chuncheongbuk-do, Korea
3Department of Food Science & Bio Technology, Shinansan University, Ansan-si, Gyeonggi-do, Korea
Corresponding author:  Young Min Kim ,Tel: +82 42 629 8760 , Fax: +82 42 629 8873, Email: kym@hnu.kr
Received: December 7, 2017;  Accepted: April 30, 2018.  Published online: June 30, 2018.
ABSTRACT
Purpose:
This study aimed to determine the anti-wrinkle and skin turnover improvement effects of niacinamide-dipeptide convergence (N-peptide).
Method:
The following methods were employed to assess the anti-wrinkle and skin turnover improvement effects of N-peptide: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, wound healing assay, chicken chorioallantoic membrane (CAM) assay, reverse transcription polymerase chain reaction (RT-PCR), and three-dimensional (3D) cell culture.
Results:
The MTT assay revealed that the N-peptide does not exert a cytotoxic effect in human keratinocytes (HaCaT). Moreover, the scratch wound healing and CAM assays showed that the N-peptide induced migratory properties and angiogenesis, respectively, in HaCaT cells. The expression of matrix metalloproteinases (MMP)-1, -2, -3, and -9 was measured using RT-PCR to evaluate the anti-wrinkle effect of N-peptides. The findings demonstrated that N-peptides were responsible for reducing the expression of the aforementioned MMPs. In addition, using western blotting, N-peptides were identified to increase energy metabolism activity. Furthermore, 3D cell culture analysis indicated improvement in the skin turnover rate because of the N-peptides.
Conclusion:
Taken together, these results suggest that N-peptides may be a source of cosmetic agents for the improvement of wrinkles and skin turnover.
Key words: Niacin-dipeptide convergence, Functional cosmetic, Anti-wrinkle, Skin barrier, Skin turnover rate
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