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Kor J Aesthet Cosmetol > Volume 10(4); 2012 > Article
Kor J Aesthet Cosmetol 2012;10(4): 949-960.
인간 피부줄기세포 배지의 개발과 이를 조성물로 제조한 화장품의 유효성 평가
최은주, 조홍범, 윤현수
Developments of Culture Media for Human Skin Stem Cell, and Evaluation of Efficacy of Cosmetics Containing Culture Media
Eun Joo Choi, Hong Bum Cho, Hyun Soo Yoon
This research evaluates the efficacy of cosmetic products manufactured by applying stem cell culture techniques. The results are obtained after analysing the characteristics of cells cultured in the medium and evaluating function of cosmetics and the effectiveness using MTS procedure. When culturing human skin cell and skin stem cell after manufacturing culture medium by mixing approved cosmetics ingredients, the growth and proliferation of cells were assured. Also associated with skin regeneration from cultured cells and cell function was remarkably improved. With the culture medium of skin stem cell, Skin essence was manufactured. This cosmetics showed outstanding improvement effect on skin condition at sensory test of skin moisturizing, anti-wrinkle, whitening at the time of its use. In addition, a clinical test was conducted to assess the effectiveness of the Skin essence by applying skin essence containing culture medium after MTS procedure used as a physical stimulation method in efficient absorption and delivery of cosmetic ingredients. After MTS procedure, application of skin essence was represented to reduce erythema amount of facial skin and significantly increase moisture content. We conclude that cosmetics including culture medium ingredients of skin stem cell promotes the regeneration of skin whitening and anti-winkle and improves the status of trouble by soothing skin irritation by MTS procedure. Futhermore, this cosmetics showed the striking cosmetic effect of improving skin condition by maintaining and increasing skin moisture.
Key words: Stem cell culture media, Cosmetics, Skin regeneration, Evaluation of efficacy
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