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Kor J Aesthet Cosmetol > Volume 12(5); 2014 > Article
Kor J Aesthet Cosmetol 2014;12(5): 719-724.
감잎추출물의 항산화 및 항염증 효과
이정희
Anti-Oxidant and Anti-Inflammatory Effects of Diospyros kaki Thumb Leaves Extracts
Jung-Hee Lee
ABSTRACT
Oxidative stress has been implicated in cutaneous damage in various inflammatory skin diseases, including psoriasis. Flavonoids have increased lately due to their broad pharmacological effect, explained by their inhibition of certain enzymes and their antioxidant activity. Accordingly, to develop oxidant and inflammation care cosmetic ingredients, the extracts Diospyros kaki leaves were selected. In this study, we investigated effect on cell viability and anti-oxidant activity of Diospyros kaki leaves extracts by using sulforhodamine B (SRB) assay and 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. The anti-inflammatory activity of Diospyros kaki leaves extracts on nitrite production and tumor necrosis factor-α (TNF-α) secretion were tested using nitric oxide (NO) colorimetric assay kit and enzyme-linked immunosorbent assay (ELISA) kit. Diospyros kaki leaves extracts showed the high cell viability over 100 % against mouse macrophage RAW 264. 7 cell at concentration of 10, 50, 100 and 500 μg/mL. Those exhibited anti-oxidant activity with dose dependent manner at concentration of 10, 50, 100 and 500 μg/mL. Nitrite production and TNF-α secretion were significantly decreased with dose dependant manner according to increment of treated concentration with Diospyros kaki leaves extracts (10, 50, 100 and 500 μg/mL) (p<.05). These results demonstrate that Diospyros kaki leaves extract possessing anti-oxidant and anti-inflammatory effects may be applicable to heal skin damage.
Key words: Inflammatory skin diseases, Diospyros kaki leaves, Cosmetic ingredients, Anti-oxidant, Anti-inflammatory
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