Asian J Beauty Cosmetol. 2012; 10(3): 515-521.
Anti-oxidative Function of Fisetin and Its Potential as an Anti-oxidant Nutri-cosmetics
The term of nutri-cosmetics refers to nutritional supplements which can support the function and the
structure of the skin. The fisetin is a flavonol which belongs to the flavonoid group of polyphenols. Although
the anti-carcinogenic property of fisetin have been widely studied, anti-oxidative function of fisetin in
HaCaT keratinocytes is unknown. In this study, we examined the in vitro chemical and cellular anti-oxidant
activities of fisetin in HaCaT keratinocytes and we further discussed its potential as an anti-oxidant nutricosmetics.
We employed scavenging assay for the 1,1-diphenyl-2,5-picrylhydrazyl (DPPH) radicals and
cellular anti-oxidant activity of fisetin was investigated in H2O2-treated HaCaT keratinocytes as judged by the
2’7’-dichlorofluorescein-diacetate (DCFH-DA)-based assay. The fisetin effectively scavenged DPPH radicals
(IC50 54 μM) when compared to the scavenging activities of L-ascorbic acid (IC50 87 μM) acid and trolox (IC50
74 μM), well-known anti-oxidants. In addition, the fisetin significantly ameliorates H2O2-induced oxidative stress
in HaCaT keratinocytes in dose-dependent manner. The fisetin showed more strong cellular anti-oxidant
activity than those of L-ascorbic acid and trolox in H2O2-treated HaCaT keratinocytes. In agreement with result
of the cellular anti-oxidant activity, we observed markedly suppressed H2O2-induced oxidative stress by the pretreatment
of 100 μM fisetin under the fluorescence microscopy analysis. These results provide the scientific
evidences for the development of skin targeted nutri-cosmetics which has anti-oxidant function.
Fisetin, Nutri-cosmetics, Anti-oxidant, HaCaT keratinocytes, Oxidative stress