Asian J Beauty Cosmetol. 2013; 11(5): 877-882.
Anti-wrinkle Effects of Converted Protopanaxatriol Prepared from Korean Red Ginseng
In order to study about human skin anti-wrinkle effect of red ginseng, red ginseng extracts and the fractions were tested to inhibition effect against MMP-2, -9, and -1 expression in human fibroblast cells by gelatin zymography and RT-PCR methods. The red ginseng samples are red ginseng extract extracted with hot water, crude saponin as butanol soluble fraction, major ginsenosides, protopanaxatrol conversions, protopanaxadiol conversions as the fractions fractionated by C18 revers phase resin and various proportions of aqueous acetonitrile. We found that propanaxatriol conversions fraction one of the red ginseng samples inhibited in a concentration-dependent manner against MMP-2, and -9 expression levels in human fibroblast cells irradiated by UV light. By HPLC analysis, protopanaxtriol conversions fraction consisted on ginsenosides Rk3, F4, Rg6, and Rh4. These results suggest that dehydrates of protopanaxatriol ginsenoside from red ginseng have an antiwrinkle activity through inhibition of MMP-2, and -9 expression in skin.
Panax ginseng, Protopanaxatriol, MMPs, Anti-wrinkle