Asian J Beauty Cosmetol. 2015; 13(4): 509-515.

Melanin Inhibitory Activity and Skin Effect of Rumex crispus L. leaf extracts in B16F10 Melanoma Cellse

By using the Rumex crispus L. leaf ethanol extract, the attempt is undertaken to measure the melanin inhibitory activity and tyrosinase inhibitory activity and cell viability in B16F10 melanoma cells, melanin inhibitory activity and the essence and pack manufactured by applying to the melanin and erythema values were also studied in a way of reviewing the function as the functional cosmetics composition. As a result, the inhibition of the tyrosinase was shown its effect at the density of 125 ug/mL of extracted material. For the B16F10 cell, the cell viability show the higher than 90% survived in concentration under 100ug/mL. The tyrosinase activation control was shown to be 8.0% at 100 ug/mL while the melanin inhibitory activity was for 13.0%. The change of the melanin and erythema value of skin was shown to have the noticeable difference (p<.001) in the experiment group following the time of use for the index prior to experiment compared to the contrast group. According to this result, the Rumex crispus L. leaf extract had affected for the skin to brighten and showed possibility material of functional cosmetics. It looks like there needs to be continuing research about this matter.

Keywords : B16F10, Rumex crispus L., Tyrosinase, Melanin, Erythema