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Kor J Aesthet Cosmetol > Volume 11(1); 2013 > Article
Kor J Aesthet Cosmetol 2013;11(1): 43-50.
정상모의 퍼머넌트 웨이브 시술 시 분자량 분포도가 다른 갯지렁이(Marphysa sanguinea ) 단백질 분해물의 전처리 효과
신지은, 강상모
Pretreatment Effect of Different Molecular Weight Lugworm Protein Hydrolyzate in Treating Permanent Wave of Virgin Hair
Ji-Eun Shin, Sang-Mo Kang
This study was intended to clarify pre-treatment effect of the lugworm autolysates of which molecular weight distribution are different respectively in treating permanent wave on normal hair. We added the protease crude enzyme extract gotten by cultivating Bacillus subtilis K-54 separated from cheonggukjang when making the lugworm autolysates. In the meantime, we made the molecular weight distribution of lugworm autolysates became different through making the additive amount of protease crude enzyme became different. We observed the molecular weight distribution of lugworm autolysates using SDS-PAGE, and measured hair thickness, tensile strength, density analysis by methylene blue dyeing, wave formation & maintenance strength with the protease crude enzyme (0.112 unit) after carrying out the pre-treatment on each autolysates. In the event of making the lugworm autolysates, the highest rate for the permanent effect was shown at MbB50 (0.8950×50 unit) of which protease per 1g lugworm was added by about 50 times. but effect was shown at MbB30 (0.0895×30 unit) in wave long-lasting. As the result of study, for securing quality as a pretreatment agent in treating permanent wave on normal hair, it was thought that it is more important to make the earthworm protein became the decompression substances with the proper size than to make them became the low molecular protein.
Key words: B . subtilis K-54, Lugworm, Protease, Protein, Virgin hair
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