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Kor J Aesthet Cosmetol > Volume 11(4); 2013 > Article
Kor J Aesthet Cosmetol 2013;11(4): 715-719.
호두 껍데기 에탄올 추출물의 항산화 및 각질형성세포에서 Tumor Necrosis Factor Alpha에 의해 유도된 Metalloproteinase-1 발현 저해 효과
Walnut Husk Ethanol Extract Possess Antioxidant Activity and Inhibitory Effect of Matrix Metalloproteinase-1 Expression Induced by Tumor Necrosis Factor Alpha in Human Keratinocyte
Jang-Soon Park
This study was evaluated antioxidant activity of ethanol extracts of Juglans regia (walnut) husk (WHE) using 1,1-diphenyl-2-picryl hydrazyl (DPPH) method. Also, cell viability of WHE on human keratinocyte (HaCaT) was evaluated by sulforhodamine B (SRB) assay. Inhibitory effect of WHE on tumor necrosis factor alpha (TNF-α) induced Matrix Metalloproteinase-1 (MMP-1 ) expression in HaCaT was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR). As the results, WHE was showed similar antioxidant activity to vitamin C at concentration of 6.25, 12.5, 25, 50 μg/mL. WHE of low concentration (6.25, 12.5 and 25 μg/mL) didn’t exhibited cytotoxicity in HaCaT. However, treatment of 50 and 100 μg/mL WHE were showed cell viability of about 98.4% and 91.1%, respectively, in same cells. Matrix Metalloproteinase-1 (MMP-1 ) mRNA were induced by TNF-α in HaCaT cells and WHE treatment of 10, 20, 50 and 100 μg/mL were decreased MMP-1 mRNA with dose-dependent manner. Exhibiting excellent antioxidant activity with high cell viability, and potent inhibitory effect on MMP-1 mRNA, ethanol extracts of walnut husk extracts can be applied in cosmetics as a MMP-1 inhibitor.
Key words: Walnut, Antioxidant activity, Cell viability, MMP-1, Cosmetics
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