Synergetic Protective Effects against Oxidative Stresses by Cotreatment of <i>Centella asiatica</i> and Follic Acid-Ferment Extracts and Phytosphingosine-1-Phoaphte in Human Dermal Fibroblasts

Lee, Ghang Tai; Lee, Kwang Sik; Cha, Hwa Jun; An, Sungkwan; Kim, Soo Young; Ahn, Kyu Joong; Lee, Kun-Kook; Lee, Ghang Tai; Lee, Kwang Sik; Cha, Hwa Jun; An, Sungkwan; Kim, Soo Young; Ahn, Kyu Joong; Lee, Kun-Kook

Kor J Aesthet Cosmetol > Volume 12(6); 2014 > Article

Kor J Aesthet Cosmetol 2014;12(6): 853-858.

인간진피섬유아세포에서 병풀(Centella asiatica)과 엽산(Follic aicd)을 주성분으로 하는 복합발효추출물과 Phytosphingosine-1-phosphate의 복합처리의 산화적 스트레스 보호 시너지효과

이강태, 이광식, 차화준, 안성관, 김수영, 안규중, 이건국

Synergetic Protective Effects against Oxidative Stresses by Cotreatment of Centella asiatica and Follic Acid-Ferment Extracts and Phytosphingosine-1-Phoaphte in Human Dermal Fibroblasts

Ghang Tai Lee, Kwang Sik Lee, Hwa Jun Cha, Sungkwan An, Soo Young Kim, Kyu Joong Ahn, Kun-Kook Lee

ABSTRACT

The present study aims to evaluate synergetic effects of co-treatment with Centella asiatica (C. asiatica) and follic acid-ferment extracts (CFFE) and phytosphingosine-1-phoaphte (PhS1P). Cosmetic effects of each of CFFE and PhS1P are relatively well-known. However, synergetic effects of co-treatment with CFFE and PhS1P are not understood currently. Therefore, we investigated synergetic effects of co-treatment with CFFE and PhS1P and how co-treatment with CFFE and PhS1P exerts its synergetic effects. First, we determined that co-treatment with specific-concentration of CFFE and PhS1P increased cell viability. At same concentration of CFFE and PhS1P, HDFs were resisted oxidative stress-dependent cell growth arrest. In addition, we show that co-treatment of CFFE and PhS1P repressed c-Jun N-terminal kinase (JNK) activation and JNK-mediated oxidative stress response in HDFs. And co-treatment of CFFE and PhS1P-mediated blocking of JNK activation induced decrease of MMP1 mRNA expression. Therefore, co-treatment of CFFE and PhS1P synergetic protects oxidative-stress through repressing JNK phosphorylation and transcriptional activity of AP-1 complex. And these results suggest that cosmetics contained with both CFFE and PhS1P may have more therapeutic effects against oxidative-stress-mediated skin aging and formation of wrinkle.

Key words: Phytosphingosine-1-phoaphte, Human dermal fibroblasts, Centella asiatica, Follic acid, Ferment extracts